Lebing Wang, Jianguo Deng, Dongbin Wang  This email address is being protected from spambots. You need JavaScript enabled to view it., Menghao Chen, Xue Li, Yun Lu, Jingkun Jiang  This email address is being protected from spambots. You need JavaScript enabled to view it. 

State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China

Received: January 31, 2022
Revised: April 23, 2022
Accepted: April 24, 2022

 Copyright The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.

Download Citation: ||https://doi.org/10.4209/aaqr.220049  

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Cite this article:

Wang, L., Deng, J., Wang, D., Chen, M., Li, X., Lu, Y., Jiang, J. (2022). An Exhaled Breath Sampler Based on Condensational Growth and Cyclone Centrifugation (BSCC). Aerosol Air Qual. Res. 22, 220049. https://doi.org/10.4209/aaqr.220049


  • A new exhaled breath sampler BSCC was developed to collect exhaled aerosol for disease diagnosis.
  • BSCC collects aerosols via condensational growth plus centrifugation.
  • Collection efficiency for 100 nm is 66.7% and 93.5% infectivity preservation is achieved.


An exhaled breath sampler based on condensational growth and cyclone centrifugation (BSCC) was developed and evaluated. The BSCC increases the size of exhaled breath aerosols through condensational growth and then collects them as liquid sample via centrifugation. This enables rapid sample collection and eliminates certain pre-treatment steps for pathogenic microorganism analysis. Laboratory-generated aerosols were mixed with saturated water vapor to simulate exhaled breath, and the collection efficiency and the virus infectivity conservation efficiency of the BSCC were evaluated. The collection efficiency of the BSCC was approximately 66.7% for 100 nm aerosols and increased to nearly 100% for 3 µm aerosols. Besides, the BSCC maintained approximately 93.5% infectivity of atomized model virus aerosol (Pseudomonas bacteriophage Phi6). When collecting exhaled breath samples from nine volunteers, the average collection rate was 248.7 µL min–1. The BSCC achieved superior overall performance (i.e., 60% high collection efficiency and 40% higher infectivity conservation efficiency) compared with RTube, a commercial used exhaled breath sampler, indicating its potential for diagnosis of respiratory infection and measurements of exhaled viral aerosols.

Keywords: Viral aerosol collection, Exhaled breath, Sampler development, Collection efficiency, Viral infectivity conservation

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