Quentin Hamdaoui1,3, Anna Bencsik2, Frédéric Flamant3, Simon Delcour1, Tatiana Macé1, Sophie Vaslin-Reimann1, François Gaie-Levrel This email address is being protected from spambots. You need JavaScript enabled to view it.1 

1 Laboratoire national de métrologie et d'essais (LNE), Paris, France
2 Université Claude Bernard Lyon 1, ANSES, Laboratoire de Lyon, France
3 IGFL, Functional genomics of thyroid hormone signaling group, Lyon, France

Received: November 12, 2020
Revised: March 11, 2021
Accepted: April 4, 2021

 Copyright The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.

Download Citation: ||https://doi.org/10.4209/aaqr.200626  

Cite this article:

Hamdaoui, Q., Bencsik, A., Flamant, F., Delcour, S., Macé, T., Vaslin-Reimann, S., Gaie-Levrel, F. (2021). Metrological Characterization of an Aerosol Exposure Chamber to Explore the Inhalation Effects of the Combination of Paraquat and TiO2 Nano-objects. Aerosol Air Qual. Res. 21, 200626. https://doi.org/10.4209/aaqr.200626


  • An aerosol exposure chamber for toxicology studies was metrologically validated.
  • Aerosols of nano-TiO2 and/or paraquat were produced as a model of nanopesticide.
  • Atmosphere stability and environment parameters were assessed in the exposure chamber.
  • Reproducibility was evaluated with a preliminary campaign on the field.


Agriculture emits a significant quantity of airborne contaminants, and the prospective environmental release of nanopesticides, a new type of agrochemical that employs engineered nanomaterials (ENMs) as either active substances or additives in a pesticide formulation, raises concerns about the risks of inhalation which are still unknown. Although the adverse effects of pesticides have been studied extensively, the potential synergistic toxicity between these substances and ENMs has rarely been investigated. To this end, toxicological models are essential to estimating the health consequences of such aerosols. Thus, to assess the respiratory hazards of titanium dioxide nano-objects (specifically, AEROXIDE® TiO2 P25 nanopowder [nTiO2]) in combination with paraquat (PQ), we developed a dynamic whole-body exposure chamber for rodents in compliance with guidelines for inhalation toxicity testing (Organization for Economic Cooperation and Development (OECD)) and animal welfare. First, we metrologically characterized the generated test aerosols by determining their mass and number concentrations, size distributions and atmospheric homogeneity at the laboratory. Then, we evaluated the reproducibility and proper functioning of the chamber during a preliminary field campaign, which validated the consistency of the aerosols’ mass and number concentrations between the laboratory characterization and the rodent exposure sessions. Finally, we examined the inhalation effects on the rodents.

Keywords: Aerosol, Inhalation paraquat, TiO2 nano-objects

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