Quentin Hamdaoui1,3, Anna Bencsik2, Frédéric Flamant3, Simon Delcour1, Tatiana Macé1, Sophie Vaslin-Reimann1, François Gaie-Levrel This email address is being protected from spambots. You need JavaScript enabled to view it.1 

1 Laboratoire national de métrologie et d'essais (LNE), Paris, France
2 Université Claude Bernard Lyon 1, ANSES, laboratoire de Lyon, France
3 IGFL, Functional genomics of thyroid hormone signaling group, Lyon, France


Received: November 12, 2020
Revised: March 11, 2021
Accepted: April 4, 2021

 Copyright The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.


Download Citation: ||https://doi.org/10.4209/aaqr.200626  


Cite this article:

Hamdaoui, Q., Bencsik, A., Flamant, F., Delcour, S., Macé, T., Vaslin-Reimann, S., Gaie-Levrel, F. (2021). Metrological Characterization of an Aerosol Exposure Chamber to Explore the Inhalation Effects of the Combination of Paraquat and TiO2 Nano-objects. Aerosol Air Qual. Res. https://doi.org/10.4209/aaqr.200626


HIGHLIGHTS

  • An aerosol exposure chamber for toxicology studies was metrologically validated.
  • Aerosols of nano-TiO2 and/or paraquat were produced as a model of nanopesticide.
  • Atmosphere stability and environment parameters were assessed in the exposure chamber.
  • Reproducibility was evaluated with a preliminary campaign on the field.
 

ABSTRACT


Agriculture is a main source of airborne contaminants and the perspective of a potential environmental release of a new type of pesticides - the nanopesticides - raises questions about the inhalation effects of these agrochemicals. These innovative products, whose inhalation effects are still not explored, encompass engineered nanomaterials (ENMs) used either directly as active substances, or mixed as ENMs additives within a pesticide formulation. If the adverse effects of pesticides are extensively studied, the potential toxic synergy involving ENMs and pesticides is rarely explored. To this end, toxicological models are essential to assess the health consequences of such aerosols. The present study reports the characterization of a new exposure chamber, which is dedicated to inhalation toxicology using rodents, in order to assess the effects of the combination of titanium dioxide nano-objects (nTiO2) and paraquat (PQ). A dynamic whole-body chamber was developed in order to comply with animal welfare and the OECD guidelines for inhalation toxicology studies. The generated aerosols were first metrologically characterized in terms of mass and number concentration, size distribution and atmosphere homogeneity. The facility reproducibility and proper functioning were then evaluated during a preliminary campaign on the field. It allowed the validation of the animal exposure sessions consistency with the metrological characterization in terms of mass and number particle concentrations. Finally, we demonstrate the suitability of this new exposure chamber to explore aerosol inhalation effects on rodents.


Keywords: Aerosol, Inhalation paraquat, TiO2 nano-objects




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