Hsiao-Chi Chuang1,2,3, Yu-Ting Yang4, Hsin-Chang Chen5, Yaw-Huei Hwang4,6, Kuen-Yuh Wu4, Ta-Fu Chen7, Chia-Ling Chen1, Ming-Kai Jhan8,9, Tsun-Jen Cheng 4,5

School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan
Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei 23561, Taiwan
Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei 10617, Taiwan
Institute of Food Safety and Health, College of Public Health, National Taiwan University, Taipei 10617, Taiwan
Department of Public Health, College of Public Health, National Taiwan University, Taipei 10617, Taiwan
Department of Neurology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei 10617, Taiwan
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan


Received: October 21, 2019
Revised: January 12, 2020
Accepted: January 13, 2020

 Copyright The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.


Download Citation: ||https://doi.org/10.4209/aaqr.2019.10.0523 


Cite this article:

Chuang, H.C., Yang, Y.T., Chen, H.C., Hwang, Y.H., Wu, K.Y., Chen, T.F., Chen, C.L., Jhan, M.K. and Cheng, T.J. (2020). Acute Effects of Pulmonary Exposure to Zinc Oxide Nanoparticles on the Brain in vivoAerosol Air Qual. Res., https://doi.org/10.4209/aaqr.2019.10.0523


HIGHLIGHTS

  • Neurotoxicity caused by PM was investigated.
  • Cytotoxicity and oxidative stress were occurred by PM.
  • PM-induced microglial activation.
  • Autophagy and apoptosis were regulated by PM.
 

ABSTRACT


Although the applications for zinc oxide nanoparticles (ZnONP) are continually increasing, the neurotoxicity of this material remains unclear. This study investigated the acute effects of pulmonary exposure to ZnONP on the brain in terms of behavioral changes, oxidative stress, inflammation, and tau and autophagy expressions using rat subjects. Based on the test subjects’ performance in a Morris water maze and an elevated-plus maze, respectively, no major alterations occurred in spatial cognition or learning ability, or anxiety. Following exposure to 10 mg kg–1 of ZnONP, we observed that the level of 8-hydroxy-2ʹ-deoxyguanosine (8-OHdG)/dG significantly increased in the hippocampus, whereas those of interleukin (IL)-1β and IL-6 significantly decreased in the cerebellum and the cortex. Additionally, microglia were activated in the hippocampus. Tau protein expression was strong in the cerebellum and the hippocampus, but no significant expression of Beclin-1, light chain 3 (LC3) II/I, or ubiquitin was detected. Our results suggest that acute exposure to ZnONP induces oxidative stress, microglia activation, and tau protein expression in the brain, leading to neurotoxicity.


Keywords: Autophagy; Central nervous system; Elevated plus maze; Morris water maze; Nanoparticle; Ubiquitin.



Aerosol Air Qual. Res. 20:-. https://doi.org/10.4209/aaqr.2019.10.0523 


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