Ta-Chih Hsiao1, Jungshan Chang2, Jia-Yi Wang2,3, Dean Wu4,5, Kai-Jen Chuang6,7, Jen-Kun Chen8, Tsun-Jen Cheng9, Hsiao-Chi Chuang 10,11,12

Graduate Institute of Environmental Engineering, National Taiwan University, Taipei 106032, Taiwan
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
Department of Physiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
Department of Neurology, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan
Department of Neurology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
School of Public Health, College of Public Health, Taipei Medical University, Taipei 11031, Taiwan
Department of Public Health, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli 35053, Taiwan
Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei 10617, Taiwan
10 School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
11 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan
12 Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei 23561, Taiwan


Received: August 8, 2019
Revised: February 6, 2020
Accepted: March 19, 2020

 Copyright The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.


Download Citation: ||https://doi.org/10.4209/aaqr.2019.08.0376 


Cite this article:

Hsiao, T.C., Chang, J., Wang, J.Y., Wu, D., Chuang, K.J., Chen, J.K., Cheng, T.J. and Chuang, H.C. (2020). Serum Neurofilament Light Polypeptide is a Biomarker for Inflammation in Cerebrospinal Fluid Caused by Fine Particulate Matter. Aerosol Air Qual. Res., https://doi.org/10.4209/aaqr.2019.08.0376


HIGHLIGHTS

  • Whole-body exposure system for in vivo experiment was established.
  • Neuroinflammation caused by PM was investigated.
  • Immune responses occurred after chronic exposure to PM1.
  • Serum NEFL polypeptide is a biomarker for PM-induced neurotoxicity.
 

ABSTRACT


Epidemiological and toxicological evidence indicates that fine particulate matter (PM2.5, particulate matter < 2.5 µm in aerodynamic diameter) causes cardiopulmonary toxicity; however, its neurotoxic effects remain unclear. The objective of this study was to investigate the role of the neurofilament light (NEFL) serum polypeptide in neurotoxicity. 6-month-old male Sprague Dawley (SD) rats were exposed to traffic-related PM1 (< 1 µm in aerodynamic diameter; 16.3 µg m–3) and gaseous pollutants (via high-efficiency particulate air, HEPA) for 3 and 6 months through a whole-body exposure system. According to our observations, the levels of interleukin (IL)-4, IL-10, and tumor necrosis factor (TNF)-α in the serum of the rats significantly increased (p < 0.05) after 3 months of exposure to PM1, whereas that of NEFL polypeptide significantly increased (p < 0.05) after 3 and 6 months of exposure. Additionally, increases in the IL-2, IL-6, IL-10, IL-17a, TNF-α, and interferon (IFN)-γ levels after 3 and/or 6 months of exposure to this pollutant (p < 0.05) were observed in the cerebrospinal fluid (CSF). In terms of their respective levels, the IL-6 correlated well with the CSF IL-2 and IL-10; the TNF-α correlated well with the CSF IL-6, IL-17a, TNF-α, and IFN-γ; and the NEFL polypeptide correlated well with the CSF IL-2, IL-4, IL-6, IL-10, IL-17a, TNF-α, and IFN-γ. In summary, systemic neuroinflammatory and immune responses in rats occurred after chronic exposure to PM1. Hence, NEFL polypeptide in serum may be a suitable biomarker for neurotoxicity caused by chronic exposure to this pollutant.


Keywords: Air pollution; Central nervous system; Neurotoxicity; Oxidative stress; Particulate matter



Aerosol Air Qual. Res. 20:-. https://doi.org/10.4209/aaqr.2019.08.0376 


Impact Factor: 2.735

5-Year Impact Factor: 2.827


SCImago Journal & Country Rank

Enter your email below to receive latest published articles in your field.