Pan Wang1, Shouqi Yuan 1, Ning Yang 2, Aiying Wang3, Alexander Fordjour1, Shengbo Chen1

Research Center of Fluid Machinery Engineering and Technology, Jiangsu University, Zhenjiang 212013, China
School of Electrical and Information Engineering, Jiangsu University, Zhenjiang 212013, China
China National Rice Research Institute, Hangzhou 310006, China

Received: August 31, 2019
Revised: November 16, 2019
Accepted: December 19, 2019
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Cite this article:

Wang, P., Yuan, S., Yang, N., Wang, A., Fordjour, A. and Chen, S. (2020). The Collection Method for Crop Fungal Spores Based on an Efficient Microfluidic Device. Aerosol Air Qual. Res. 20: 72-79. doi: 10.4209/aaqr.2019.08.0424.


  • An ultra-compact microscale system was designed to provide good portability.
  • A novel pretreatment was proposed to achieve a satisfied sharpness.
  • The low-pressure collection chamber eliminates particle bounce and re-entrainment.


Nowadays, the airborne fungal spores have always taken an important role in the spread of crop fungal diseases and caused great concern. In this study, a novel efficient microfluidic chip for enriching the airborne fungal spores directly from gas flow was developed, which has better portability and cost-efficiency. The chip consists of three parts: half-wave pretreatment channel, inertial impactor, and low-pressure collection chamber. During the collection process, the particles were arranged in the radial position of the half-wave pretreatment channel based on their sizes, then separated by the inertial impactor and collected in the low-pressure collection chamber. The size distribution of the collected target was calculated by image process and recognition. The results show that the cut-off sizes of the proposed microfluidic device was found to be 4.83 µm (first-stage) and 0.98 µm (second-stage), respectively. The sharpness of the first and the second-stage collection efficiency curves were 1.31 and 1.79 respectively. The bounce effects and re-entrainment of particles can be eliminated by the low-pressure collection chamber without any silicon oil or grease. The collection reproducibility is acceptable. As a result, the proposed microfluidic chip can realize the crop fungal spore collection efficiently and can be used to improve the development of real-time crop fungal diseases monitoring technology.

Keywords: Crop fungal spores; Microfluidics; Separation performance; Pretreatment.

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