Hyeong-U Kim1, Junhong Min2, Geunsang Park3, Dongjoo Shin3, Giwoon Sung3, Taesung Kim 1,3, Min-Ho Lee 2


SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 440-746, Korea
School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea
School of Mechanical Engineering, Sungkyunkwan University, Suwon 440-746, Korea



Received: July 3, 2018
Revised: September 4, 2018
Accepted: September 10, 2018
Download Citation: ||https://doi.org/10.4209/aaqr.2018.06.0221  


Cite this article:
Kim, H.U., Min, J., Park, G., Shin, D., Sung, G., Kim, T. and Lee, M.H. (2018). Electrochemical Detection of Airborne Influenza Virus using Air Sampling System. Aerosol Air Qual. Res. 18: 2721-2727. https://doi.org/10.4209/aaqr.2018.06.0221


HIGHLIGHTS

  • Custom-made air sampler system and electrochemistry were developed and measured.
  • The air sampler was designed by numerical prediction depending on flow rate.
  • Collection efficiency was evaluated with controlled injection of target antigens.
  • Influenza antigen (H1N1) is injected into air chamber for sampling virus test.
  • The Au-PCB conjugated with pair antibodies showed higher response than Au-PCB.

ABSTRACT


Sampling and pre-detecting infectious diseases are effective ways of preventing them from widely spreading. Among the conventionally used sampling methods, cyclone-based air sampling is considered the simplest and most effective. In this study, we developed a new cyclone-type air sampler device by modifying the size and shape of a commercially available product, Coriolis µ (Bertin Technology). The newly built air sampler’s collection efficiency was measured using polystyrene particles by comparing the amounts before and after sampling. Additionally, to test the feasibility of sampling airborne viruses, recombinant influenza virus antigen (H1N1) was injected into the custom-built air chamber where the air sampler’s inlet was connected so that the antigen was collected through the sampling process. The solution was measured using a custom-made electrochemical platform that consisted of antibody conjugated Au electrodes on printed circuit board (PCB) and a small size reading system. Our findings showed that the influenza antigen was collected with good efficiency as well as detected with high sensitivity.


Keywords: Airborne; Virus; Sampler; Cyclone; Influenza

 



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