In the EU, there is an increasing need for regulatory agencies to establish health-based threshold limits for airborne particles. A prerequisite for such projects is the validation and comparison of existing and newly developed particle analyzers. Corresponding proficiency tests have often been conducted with the help of inter-laboratory tests using test aerosols. Although test aerosols in the micro- and the nanoscale range were produced with different generator systems at the technical center of the Institute for the Research on Hazardous Substances (IGF) in Dortmund, a stable and reproducible method of producing a low number concentration for nano-sized particles was not achieved. Inspired by a method of monodisperse droplet generation, we coupled a flame generator with a droplet generator and examined the concentration and the diameter of the formed particles as a consequence of the precursor concentration and the droplet frequency. In addition, the reproducibility of the method was tested daily, and the nanoparticles were collected and characterized microscopically. Finally, the measurements of the particle size distribution were mathematically examined. The resulting fits enable the prediction of the median particle diameter as a function of the precursor concentration and the frequency of the droplets. Overall, the performed experiments confirm that this system meets all the requirements with regard to a low number concentration and long-term stability and reproducibility and should therefore be suitable for further inter-laboratory round robin tests.