This paper reports the results of a study related to the optical and physical characteristics of columnar aerosols and variation in total column ozone (TCO) and precipitable water content (PWC) over Gadanki (13.45°N, 79.18°E), a tropical station in peninsular India, for the first time, using MICROTOPS-II (Microprocessor-based Total Ozone Portable Spectrometer), comprising of both sun photometer and ozonometer. Results show wavelength dependence of AOD, having mean value of ~0.4 (± 0.09) at 500 nm optical channel. Daily mean aerosol size spectra shows, most of the time, power-law distribution. However, its diurnal variations show significant changes in aerosol size spectra modulated by a combination of both power-law and bi-modal distributions. To characterize AOD, the Ångström parameters (i.e., α and β) were used. The day-to-day variations in TCO were found to be in fair agreement with that derived from TOMS satellite data for all the experimental days, having mean observed value of ~253 (± 8) DU over the station. Interestingly, an inverse relationship between TCO and AOD or PWC was observed over the station, on some times of the day, which could be attributed to the mixing of significant fraction of ozone with aerosol and water vapor-rich air mass. However, a significant positive correlation was observed between AOD and PWC.